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Centre de Formation Continue en Santé et Sciences de la vie Université libre de Bruxelles FC-686

IMAGE-3.3 - introduction to fluorescence microscopy - sample preparation, microscopes, and image analysis

IMAGE-3.3 - introduction to fluorescence microscopy - sample preparation, microscopes, and image analysis

Short presentation

Acquire knowledge in processing and analysis of fluorescence microscopy images

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Call to actions

  • Programme title
    IMAGE-3.3 - introduction to fluorescence microscopy - sample preparation, microscopes, and image analysis
  • Programme mnemonic
    FC-686
  • Programme organised by
    • Centre de Formation Continue en Santé et Sciences de la vie
    • Université libre de Bruxelles
  • Title type
    formation continue
  • Open to returning students
    yes
  • Schedule type
    Daytime
  • Languages of instruction
    english
  • Programme duration
    short (2 to 5 days)
  • Category / Topic
    Health - Biomedical and pharmaceutical sciences

Presentation

Details

General information

Title type

formation continue

Programme duration

short (2 to 5 days)

Learning language(s)

english

Schedule type

Daytime

Category(ies) - Topic(s)

Health - Biomedical and pharmaceutical sciences

Organising faculty(s) and university(ies) Open to returning students

yes

Tarifs

  • Job seeker rate: € 0.00
  • ULB doctoral student rate (possibility of obtaining a grant): € 150.00
  • Doctoral student rate other university: € 300.00
  • Other public rate: € 500.00
  • Industrial tarif: 700,00 €

Formation continue

Speakers

  • Louise Conrard, PhD (ULB, CMMI)
  • Egor Zindy, PhD (ULB, CMMI)
  • Maud Martin, Pr (ULB, CMMI)
  • Olivier Debeir, Pr (ULB)

Contacts

+32 71 37 86 96

helsci@ulb.be

Partner

Presentation

  • To obtain useful fluorescence images by optimizing sample preparation and understanding the microscope’s important elements and acquisition parameters
  • To discover the various fluorescence microscopes and their specific domain of application
  • To learn concepts and go-to methods for processing, analysing and quantifying images that were acquired with fluorescence microscopes
  • To get familiar with ImageJ et CellProfiler, to learn how to use them, from basic usage to more advanced topics such as processing of image series and macro coding

Feel free to bring your own challenges in terms of samples or images to analyse

The course focuses on fluorescence microscopy images in life science, but the image processing concepts taught can be applied to any other scientific field.

Daytime

  • 2-day course- from 9:15 till 17:00

Calendar & registration

Target audience

  • Biologists, researchers, technicians, and students wishing to use -or already using- fluorescence microscopy to answer biological questions, regardless to their specific field
  • Staff working on bio-imaging platforms wishing to extend their knowledge.
  • IT professionals, developers, image analysis specialists that are not yet familiar with software used in life sciences

Candidates will be selected based on their profile and the maximum number of seats available


 

Calendar & registration

Programme

PART 1: Learn the bases of fluorescence microscopy

  • Which microscope should I use (to answer a specific biological question)?
  • Sample preparation
  • Microscopes operation and important image acquisition parameters
  • Hands-on microscopes demonstration using Zeiss software or micro-manager

PART 2: Image processing and quantifications

  • Concepts and methods of image processing:
  • Bit depth, histogram adjustment, denoising and filtering, registration
  • Concepts and methods of image analysis: Segmentation, tracking an object in image series, co-localisation of fluorescent objects
  • Demo and exercises with ImageJ for routine image processing tasks, and CellProfiler for automated analyses and quantifications
  • Overview of other useful software