Generation of lung and thyroid organoids from human embryonic stem cells [Offre de technologie]

New process for creating thyroid and lung tissues from stem cells, achieving all cell types from both organs with high efficiency, while cells autonomously organize into organoids.

Using a hESC line, we have generated a protocol where by using insulin we can switch differentiation of human embryonic stem cells from thyroid fate towards lung fate. In this protocol we used a human embryonic stem cell line genetically modified to overexpress two main thyroid transcription factors, NKX2.1 and Pax8 when treated with doxycycline.

• For thyroid model, the technology efficiently produces the single cell type of the thyroid, organizing them into follicles akin to the native organ.
• Modifiable culture conditions for lung model, enabling the promotion of specific cell types, such as alveolar cells, crucial for gas exchange.
• The technology enables the efficient production of all cell types from both organs, with resulting cell populations self-organizing structurally into organoids.

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IRIBHM is part of the Medical School of the Université Libre de Bruxelles (ULB) and one of the largest research structures of the university. Founded in the 1960s with the aim of applying an interdisciplinary approach to the study of thyroid pathophysiology, the Institute has thrived over the years into a number of independent groups with diversifying research interests. Presently, about 130 researchers and technicians are working in the Institute over a range of subjects encompassing signal transduction, development, neuroscience and cancer, using cell and molecular biology approaches. Staff researchers include physicians, physicists, bioinformaticians, (bio)chemists and biologists.

The activities of IRIBHM are mainly taking place on the Erasme campus of ULB, in the suburbs of Brussels, although the Institute also contributes to the Institut de Biologie Moléculaire et Médicale (IBMM) on the Gosselies campus. The heavy equipment is common to the whole Institute and often shared with other groups of the campus as technological platforms. This includes genomics, proteomics, transgenesis, FACS and confocal microscopy facilities. 

• Transplantable human thyroid organoids generated from embryonic stem cells to rescue hypothyroidism. Mírian Romitti, Adrien Tourneur, Barbara de Faria da Fonseca, Gilles Doumont, Pierre Gillotay, Xiao-Hui Liao, Sema Elif Eski, Gaetan Van Simaeys, Laura Chomette, Helene Lasolle, Olivier Monestier, Dominika Figini Kasprzyk, Vincent Detours, Sumeet Pal Singh, Serge Goldman, Samuel Refetoff, Sabine Costagliola.

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